rabbit anti p44 42 mapk  (Cell Signaling Technology Inc)

Journal: Frontiers in Plant Science

Article Title: Discovery that PACAP, a mammalian neuropeptide, activates plant immunity through chemical screening

doi: 10.3389/fpls.2026.1787727

Figure Lengend Snippet: PACAP functions as an elicitor to trigger plant immune responses. (A) Quantification of calcium influx in Col-Q seedlings expressing aequorin. Luminescence signals were recorded for a period of 30 min following treatment of Col-Q seedlings with PACAP 1-38, PACAP 6-38, or PACAP 1–27 at concentrations of 20, 50, and 100 μM, as indicated. Seven-day-old seedlings were preloaded overnight with coelenterazine before measurement. Values are means ± SEM (n = 8 biological replicates). Col-Q: Col-aequorin. (B) Calcium ion influx was measured in aequorin transgenic seedlings for 30 min after treatment with mock (sterile water), 100 nM flg22, 50 μM PACAP 1-38, 50 μM PACAP 6-38, or 50 μM PACAP 1-27. (C) PACAP induces MAPK activation. Arabidopsis seedlings were soaked in 50 μM PACAP 1-38, 50 μM PACAP 6-38, or 50 μM PACAP 1–27 for the indicated times, followed by immunoblotting with an anti-p44/p42 antibody. 100 nM flg22 served as a positive control. Ponceau S staining served as the loading control. (D) PACAP 1–38 induces the expression of defense-related marker genes FRK1 and WRKY29 . Col-0 seedlings were soaked in 25 μM PACAP 1–38 for 0, 2, 4, and 6 h before analysis of FRK1 and WRKY29 transcript levels by RT-qPCR. Flg22 treatment served as a positive control. Values are means ± SEM (n = 3 biological replicates). * p < 0.05, ** p < 0.01, *** p < 0.001(two-tailed Student’s t test).

Article Snippet: Equal amounts of protein (14 μL per lane) were subjected to SDS–PAGE using 4–20% gradient gels and transferred to PVDF membranes (Millipore) at 100 V for 1 h. Phosphorylated MPK3 and MPK6 were detected using rabbit monoclonal anti-phospho-p44/42 MAPK (Erk1/2) antibody (Cell Signaling Technology, Shanghai, China; 1:2000).

Techniques: Expressing, Transgenic Assay, Sterility, Activation Assay, Western Blot, Positive Control, Staining, Control, Marker, Quantitative RT-PCR, Two Tailed Test

Journal: Scientific Reports

Article Title: Delivery of lipid nanoparticles containing small interfering RNA targeting transmembrane serine protease 4 in a human gastric cancer model using nude mice

doi: 10.1038/s41598-025-32407-x

Figure Lengend Snippet: In vitro silencing of transmembrane serine protease 4 (TMPRSS4) suppresses ERK1/2 signal in NUGC-3 gastric cancer cells. ( a ) Western blot comparing TMPRSS4, phospho-p44/42MAPK, p44/42MAPK, and β-actin. ( b ) Phosphorylation of level of MAPK. Band density measured using Image J. * p < 0.05. n = 3. C: control- small interfering RNA (siRNA); T: TMPRSS4 si-RNA.

Article Snippet: For phosphor-p44/42 MAPK (Erk1/2) rabbit monoclonal antibody (1:2000, D13.14.4E, Cell Signaling Technology), cells were washed with PBS and lysed with RIPA buffer (ThermoFisher Scientific, Waltham, MA) containing protease and phosphatase inhibitor cocktail (ThermoFisher Scientific, Waltham, MA).

Techniques: In Vitro, Western Blot, Phospho-proteomics, Control, Small Interfering RNA